RRM2 monoclonal antibody (M01), clone 1E1
产品名称： RRM2 monoclonal antibody (M01), clone 1E1
英文名称： RRM2 monoclonal antibody (M01), clone 1E1
产品编号： H00006241-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant RRM2.
- Immunogen:
- RRM2 (NP_001025, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MLSLRVPLAPITDPQQLQLSPLKGLSLVDKENTPPALSGTRVLASKTARRIFQEPTEPKTKAAAPGVEDEPLLRENPRRFVIFPIEYHDIWQMYKKAEASFWTAEEVDLS
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- KRAS-mediated Up-regulation of RRM2 Expression Is Essential for the Proliferation of Colorectal Cancer Cell Lines.
Yoshida Y, Tsunoda T, Doi K, Tanaka Y, Fujimoto T, Machida T, Ota T, Koyanagi M, Takashima Y, Sasazuki T, Kuroki M, Iwasaki A, Shirasawa S.Anticancer Research July 2011 vol. 31 no. 7 2535-2539
- 2.
- Epstein-Barr virus episome stability is coupled to a delay in replication timing.
Zhou J, Snyder A, Lieberman PM.J Virol. 2009 Mar;83(5):2154-62. Epub 2008 Dec 10.
- 3.
- Quantitative phosphoproteome profiling of Wnt3a mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine-20 in canonical Wnt signal transduction.
Tang LY, Deng N, Wang LS, Dai J, Wang ZL, Jiang XS, Li SJ, Li L, Sheng QH, Wu DQ, Li L, Zeng R.Mol Cell Proteomics. 2007 Nov;6(11):1952-67. Epub 2007 Aug 12.
- Applications
- Western Blot (Cell lysate)
- RRM2 monoclonal antibody (M01), clone 1E1. Western Blot analysis of RRM2 expression in HepG2 ( Cat # L019V1 ).
- Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to RRM2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to RRM2 on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of RRM2 transfected lysate using anti-RRM2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RRM2 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged RRM2 is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 6241
- GeneBank Accession#:
- NM_001034
- Protein Accession#:
- NP_001025
- Gene Name:
- RRM2
- Gene Alias:
- R2,RR2M
- Gene Description:
- ribonucleotide reductase M2 polypeptide
- Omim ID:
- 180390
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq
- Other Designations:
- ribonucleotide reductase M2 subunit